Researchers developing new multiplex protein assays need the ability to easily customize panel content to accommodate the specific clinical question under study and the ability to incorporate novel biomarkers with existing clinically relevant assays to speed the validation process. To greatly simplify these requirements, the panelPlus Sensors for the dotLab System uses a unique approach to addressing reagents.
Each assay location within the sensor is provided precoupled with a unique oligonucleotide-addressing reagent. Capture reagents are supplied pre-addressed with the complementary oligonucleotide sequence. When introduced to the sensors, they rapidly hybridize to their location without cross reacting to other spots. Simple conjugation kits allow users to address their own affinity reagents as required. This approach gives complete flexibility in designing panels and is made practical by the removal of endpoint restrictions on the assays.
Figure 2 illustrates the use of these self-addressing reagents to create a custom triplex tumor marker panel. If desired, the sensors can be regenerated by chemical disruption of the address pair and automatically replaced with a fresh capture surface. This eliminates the need to optimize regeneration for each assay and maximizes analytical performance.