The images and techniques described in this article show the utility of the Vevo 2100 High Resolution Ultrasound Imaging System as a tool for in vivo imaging and quantification of tumor angiogenesis. The noninvasive nature of ultrasound imaging allows the same tumor to be studied over the course of an experiment, leading to much stronger data and requiring fewer animals to get significant results.
The use of nontargeted MicroMarker contrast agents provides information on the vascularity of the tumor down to the capillary level. Molecular information about the expression of specific endothelial cell surface targets, such as VEGFR2, can be obtained by using the target-ready MicroMarker contrast agents.
The techniques discussed allow for acquisition of real-time data on actively perfused vessels, and therefore, would easily allow for the study of acute or chronic changes in perfusion or expression of specific markers in response to a potential therapeutic or experimental procedure.