IgY Immunoaffinity Partitioning
Beckman Coulter (www.beckmancoulter.com) offers a unique line of low-abundance protein enrichment chemistries, offering results on a multimilligram scale. The ProteomeLab IgY-12 spin and LC column kits selectively partition 12 highly abundant proteins found in plasma or serum, enriching the sample up to 25-fold. Multiple formats allow sample processing from 20 to 250 microliters per cycle.
The IgY enrichment chemistry has been optimized for human/primate and rodent samples. But one of the key attributes of IgY is its ability to partition orthologous proteins across species, allowing animal models and human samples to be processed using the same chemistry. (Figure 1)
This partitioning approach differs from protein depletion methods because it allows the capture and subsequent analysis of highly abundant proteins, if desired. Depletion methods automatically discard the bound material. Soluble biofluids, such as serum, plasma, CSF, urine, nipple aspirate, bronchial alveolar lavage, lymphatic fluid, follicular, ascites, tears, saliva, etc., are studied more clearly when the 12 highly abundant proteins are partitioned and the remaining medium- to low-abundance protein targets are highly enriched.
The IgY partitioning method has been applied at an industrial scale for in-line processing of hundreds of samples1. Several single component IgY antibody spin column kits are also available for researchers analyzing isoforms or biomolecules physiologically bound to highly abundant proteins, such as human, rat, or bovine albumin, IgG, Transferrin, Fibrinogen, or HDL.