Biological samples are highly heterogeneous, diverse, and complex. Virtually all analysis requires some type of sample preparation, yet analytical scientists often overlook the importance of this step. Ideally, sample preparation should be straightforward, reproducible, and capable of releasing all relevant analytes while preserving their structural integrity and biological activity.
Conventional biological sample preparation methods include mortar and pestle grinding, sonication, rotor-stator homogenization, French press, bead beating, freezer milling, enzymatic digestion, and chemical dissolution. All suffer from variability due to the method itself or operator inconsistencies. Moreover, they can result in destruction or incomplete release of analytes.
Pressure cycling technology (PCT) uses alternating cycles of hydrostatic pressure between ambient and ultrahigh levels (approximately 35,000 psi) to release target molecules safely and reproducibly from a wide variety of biological samples.
A PCT system consists of a Barocycler™ instrument, which generates alternating levels of hydrostatic pressure, and single-use sample processing containers. Pressure, pressure chamber temperature, and duration are under operator control. Pressure BioSciences has developed several kits for specific sample types and downstream analysis applications.