Sidebar: AACR New Product Report
> Life Technologies introduced the Applied Biosystems ViiA™ 7 RT-PCR system, which integrates quantitative PCR and genotyping applications on one platform. The system’s software can integrate real-time PCR applications such as nucleic acid quantification, SNP detection, and copy number variation analysis with applications such as high-resolution melting, proximity ligation technology for protein analysis, and gene expression without RNA purification.
Within the ViiA 7, the OptiFlex system allows users to detect up to 21 colors to enable multiplexing, and to run TaqMan and SYBR-based assays in the same run. Company officials said it also provides single-copy detection sensitivity.
> 454 Life Sciences, a Roche company, previewed the GS Junior sequencing system, set to launch this month. The GS Junior incorporates the long-read GS FLX Titanium chemistry of the Genome Sequencer FLX in a benchtop, lower-cost format.
The company also announced upcoming improvements to its Genome Sequencer FLX, including enhanced software, microfluidics, library construction, and sample preparation, that will reportedly allow for read lengths of up to 1,000 base pairs.
> Scientists at Solulink said they developed a crosslinking chemistry capable of linking all types of molecules, which retain their functionality when combined. The company’s Antibody-Oligonucleotide All-in-One conjugation kit links oligos of 20–60 base pairs in length to an antibody, creating an entity with dual functionality, according to the firm.
The technology can also be used to immobilize molecules on the surface of arrays, to link enzymes to other types of molecules, or to do dye labeling. The controlled linking process allows molecules to be joined in any desired ratio. The linker has a signature wavelength.
Solulink products include ChromaLink™ biotinylation kits, crosslinking and antibody labeling kits, and NanoLink™ and MagnaLink™ strepatvidin-coated magnetic beads in 0.8 µm and 2.8 µm sizes.
> NanoString Technologies unveiled the nCounter® miRNA expression assay kits at AACR, which are capable of highly multiplexed, direct digital detection and counting of miRNAs in a single reaction without amplification, according to the company. The kits are compatible with the nCounter analysis system workflow. The nCounter Human miRNA expression assay kit contains 736 human and human-associated viral miRNAs, which are ligated unique oligonucleotide tags to enable selective and specific detection, added a NanoString official.
> GE Life Sciences presented the NanoVue Plus UV/visible spectrophotometer with predefined methods for quantifying nucleic acids and proteins. It provides measurements in sample volumes as small as 0.5 µL to 5 µL and utilizes a sample plate that eliminates the need for cuvettes or capillaries.
The company also showcased its Mag Sepharose™ platform of magnetic Sepharose beads. The platform uses Protein A, Protein G, or NHS ligands to immunoprecipitate target proteins, or TiO2 to capture phosphorylated peptides from digested protein samples.
> Thermo Fisher Scientific featured its Solaris™ qPCR gene-expression assays and highlighted their applications in cancer research, such as the ability to use qPCR to detect members of the AKT protein kinase family in an RNAi-based study of FOX01 regulation.