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Jan 15, 2009 (Vol. 29, No. 2)

Optimization of Binding Interaction Studies

New Approach Advocates Interspot Referencing Using Multiplexed SPR Arrays

  • Conclusion

    Interspot referencing using multiplexed SPR arrays to study binding interactions can produce kinetic data that are as accurate as that generated using channel referencing, allowing all interaction spots on the array to be used. The resulting increase in throughput can be critical to such large-scale applications as screening of hybridomas supernatants, evaluating large numbers of drug lead candidates, or optimization of affinity protein purification methods. 



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