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June 15, 2011 (Vol. 31, No. 12)

Nudging Difficult-to-Express Proteins

Emerging Solutions Aim to Bring Challenging Targets Under Control

  • Some proteins are easier to express than others. For some, just clone them into an E. coli vector and let the bacteria do the work. But for others—such as toxic proteins, membrane proteins, glycosylated proteins, and hydrophobic proteins—getting them to express, fold, and function or crystalize can present a ...

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