While the Biovest HF Primer is useful for experimental needs, more sophisticated, automated, and higher production capacity units such as the AutovaxID (Figure 3) are needed for large-scale virus production. Since this system is functionally closed, yet automates mammalian cell processes, it is ideal for the production of viruses or cell-secreted proteins. A combination of pore-size selection and control of the fluid dynamics within the bioreactor encourages removal of harmful cytokines and virus-induced host shutoff proteins while maintaining virus stability.
Most importantly, the two-component design of the AutovaxID provides containment that minimizes the need for traditional cleanrooms, greatly reduces the risk of process contamination, and provides a small footprint that facilitates adaptation to existing BSL-2 or BLS-3 facilities.
AutovaxID is an example of how advanced hollow fiber technology can play an integral role in the manufacture of vaccines. In comparison to error-prone manual techniques or minimally customizable large-tank systems, the AutovaxID is:
- cGMP compliant and compact to minimize size requirements for manufacturing facilities,
- composed of disposable elements for all product contact components to maximize speed and manufacturing flexibility,
- self-contained, or functionally closed for avoidance of contamination and containment of infectious agents,
- automated to reduce operator intervention and expertise/skill levels, and
- designed to regulate perfusion rates for control of culture environments to enhance virus replication and stability.