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Oct 1, 2009 (Vol. 29, No. 17)

Novel Approaches Simplify Sample Prep

More Efficient Ways of Collecting and Concentrating Samples Are Being Introduced

  • Bead-Beating Technology

    “Sample prep is a crucial step for good results and good yields,” noted Romain Verollet, product manager at Bertin Technologies. “For toxins and pathogens, the key is to increase detection limits and linearity, which enables lower concentration detection and an easier decision-making process. Automation and standardization also increase reliability in the final analysis.”

    At the symposium, Verollet’s colleagues presented studies that investigated mechanical lysis with the Precellys bead-beater versus manual, chemical, or sonicator methods. The Precellys system enables sample preparation in 0.5 mL, 2 mL, and 7 mL in a few seconds within a temperature-controlled environment, according to the Bertin scientists.

    A high-speed multidirectional motion shakes the beads that grind and homogenize samples in sealed tubes. The system can be operated in multiple configurations in the same built-in tube holder, and it eliminates inefficient sample preparation such as a vortex, mortar and pestle, sonicator, or blender,” added Verollet.

    Three applications were examined and reviewed at the meeting—uses of DNA/RNA extraction from microorganisms (such as Bacillus), DNA extraction from plants, and protein extraction from animal tissues. “Precellys can extract, detect, and quantify DNA, RNA, or proteins,” Verollet said. In these three case studies, bead-beating technology was shown to be efficient and reproducible without sacrificing time and labor.



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