The labeled cells expressing CXCR4 were incubated with a large excess of internalization reagent and different concentrations of SDF1. A dose-response curve showing the internalization process was observed in the presence of SDF1. The same assay was performed by using 12G5 antibody, but no internalization of the receptor was observed in these conditions.
Tag-lite technology offers a lot of flexibility in terms of assay format for detecting antibody binding on cell-surface receptors. This enables the detection of different binder types, the determination of their binding affinity, as well as their ranking.
Tag-lite is HTS friendly and highly miniaturizable. It also has been shown to screen therapeutic antibodies in a cost-effective way. Moreover, frozen prelabeled Tag-lite cells can be stored and used for daily experiments and HTS campaigns. The implementation of Tag-lite assay is straightforward, and the technology has a broad application potential in the field of biotherapeutic screening and characterization, from the early R&D stage, through to production and quality control phases.