Innovative Hypoosmolar Buffers
Eppendorf (www.eppendorfna.com) offers its Multiporator for the transfection of animal and human cell lines, embryonic stem cells, primary cells, plant protoplasts, bacteria, and yeasts.
This model is compact, portable, and easy to operate, according to Joel Lopez, product manager. Our clients not only get high transfection efficiencies but also great reproducibility. We have taken a careful look at plastics, electrodes, and buffers. All of these can affect outcome.
We offer reagents and cuvettes that are optimized for our own system. This is an advantage. Most companies offer only the instrument and maybe cuvettes. Our buffers are specially developed to feature low conductivity and low osmolarity. The low conductivity boosts cell survival, allowing use of reduced voltage. Additionally, the low osmolarity of the buffer enhances pore formation.
Lopez suggests that the design of the instrument offers another advantage in that the Multiporator uses soft pulse technology. This provides for exponential decay of pulses rather than the traditional square wave pulsing. Exponential decay is like a gentle slope that dissipates over time. Overall, this is a much more cell friendly approach.
Invitrogen (www.invitrogen.com) offers transfection reagents based on its cationic lipid formulations. The company’s flagship product, Lipofectamine 2000, provides an easy protocol that works efficiently for plasmids and RNAi transfections. Customers like this product, because it works reliably on a broad range of cell lines, explains Henry Chiou, Ph.D., research manager for transfection.
We also offer products with different chemistries and formulations for specialized applications. For example, Oligofectamine is used for oligonucleotide transfections.
Invitrogen is launching several new products this year, according to Dr. Chiou. These are next-generation reagents. A limitation of current technologies is the requirement for optimizing cell densities and reagent dose. We’ve developed new reagents that expand the transfection sweet spot for more applications. These products expand the optimal working range for both reagent dose and cell density. For example, Lipofectamine LTX is designed for plasmid, shRNA, and miRNA vectors. Lipofectamine RNAiMAX is designed for short RNAi duplexes. These new reagents provide high transfection efficiency and lower cytotoxicity.
We are also introducing the Freestyle MAX Expression System for rapid protein production in suspension CHO and HEK293 cells. There is huge interest in transient transfection in suspension culture to produce large amounts of protein. This system provides all components for this application to ensure optimal compatibility, including serum-free medium, transfection reagent, and CHO and HEK293 cells, adapted to serum-free suspension culture.