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Noninvasive Oxygen and pH Sensors

Principles and Applications of Disposable Equipment in Bioprocess Development

• Sarina Arain, Ph.D.

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Figure 2. Comparative dissolved oxygen (DO) monitoring in a bioreactor with CHO cells for three weeks—showing effect of medium change on DO concentration. Blue–Competitor A electrochemical DO; red–PreSens optical DO (A). Comparative monitoring of dissolved carbon dioxide (dCO2) in a CHO-cell bioreactor culture for three weeks–PreSens optical patch sensor vs. offline electrochemical sensors. Note the close trend between real-time and standard electrochemical off-line dCO2 probes (B). Click Image To Enlarge +

Figure 2. Comparative dissolved oxygen (DO) monitoring in a bioreactor with CHO cells for three weeks—showing effect of medium change on DO concentration. Blue–Competitor A electrochemical DO; red–PreSens optical DO (A). Comparative monitoring of dissolved carbon dioxide (dCO2) in a CHO-cell bioreactor culture for three weeks–PreSens optical patch sensor vs. offline electrochemical sensors. Note the close trend between real-time and standard electrochemical off-line dCO2 probes (B).

To evaluate the efficiency of the chemical optical technology, tests were carried out comparing the chemical optical sensors with conventional electrochemical systems (Figures 2A, 2B). Measurements of DO and CO₂ were taken over a period of three weeks in a CHO cell culture. The optical sensor trended very closely with the standard electrochemical probes. Sensitivity of the optosensoric technology is higher than that of the conventional system.

PreSens’ optosensoric technology reviewed in this article can significantly contribute to improving the reproducibility and quality of cell cultivation and be applied in a broad range of scientific research.