Target enrichment is useful when a researcher is interested in sequencing a particular genome segment. Agilent’s SureSelect platform can capture a subset of exons or other genome targets and wash away the rest of the genome prior to sequencing. SureSelect replaces other labor-intensive methods of targeted re-sequencing such as PCR techniques that cause major bottlenecks in most NGS workflows, according to Dr. Ernani.
Consequently, Agilent recently introduced the SureSelect DNA Capture Array “as a way to do smaller scale target enrichment,” he says. “This complements Agilent’s in-solution SureSelect Target Enrichment System, designed for a broad range of NGS study sizes including automated high-throughput workflows.”
The Agilent SureSelect DNA Capture Array was the result of work done in collaboration with the Gregory Hannon lab at Cold Spring Harbor Laboratories. Probes were designed through eArray, Agilent’s web-based design application, and high-fidelity microarrays were then custom synthesized using the SurePrint fabrication platform.
In addition, Dr. Ernani notes that Agilent acquired Velocity 11, now called Agilent Automation Systems, and is integrating these new capabilities into the next-generation workflow, particularly the BRAVO system. Agilent also provides tools for quality control of samples using the microfluidic 2100 Bioanalyzer with its High Sensitivity DNA Kit.
“This combination allows our customers to use less PCR in their target enrichment strategy, thus reducing potential for sequencing bias,” explains Dr. Ernani.