Go For the Gold
IBIS Technologies showed off its IBIS MX96 surface plasmon resonance (SPR) system for label-free imaging of biomolecular interactions. The instrument is designed to work in conjunction with the company’s Continuous Flow Microspotter (CFM), which uses a network of microchannels to print arrays on the surface of sensors from dilute 70 µL ligand samples, said Richard Schasfoort, Ph.D., CSO.
Sensor chips with standard or custom dimensions are available from the company, with surfaces ranging from bare gold to a variety of capture surfaces and activation chemistries. Typical applications range from kinetic rate and affinity constant measurements of antigen-antibody pairs, to antibody selectivity testing and even identification of individual epitopes involved in antibody binding.
The latest system developments allow for a higher throughput and at least 20-fold greater sensitivity than IBIS’ earlier platforms, said Dr. Schasfoort. By exploiting its multiplexing capabilities, for example, 10,000 sensorgrams can be obtained overnight, which affords significant productivity increases.
This multiplexing can be used to measure a given interaction using multiple different ligand densities and analyte concentrations in a single experiment, and the software can calculate highly accurate affinity constants in a way that avoids inaccuracies typically imposed by ligand re-binding effects as encountered with conventional platforms. For customers who choose not to purchase their own system, IBIS also provides its services as a CRO.