Rapid and sensitive assays for cellular receptors are in demand for both clinical and basic science applications. Cisbio has developed a line of products based on FRET.
Tag-lite assays use a relatively undifferentiated cell line that provides a wide-ranging foundation for measuring cellular function, according to the company. The SNAP-tag labeling procedure (New England Biolabs) is used to couple a cryptate fluorophore to the receptors on the cell surfaces. Addition of a ligand carrying the second fluorophore will result in a powerful signal, forming the basis of this homogeneous assay, which requires no washing, much like ELISA-based procedures.
Tag-lite has been engineered for highly selective ligand binding, receptor dimerization, and functional assays. Among recently developed products are Cellul’erk, for measuring phosphorylated-ERK1/2, and the IP One Tb assay, for detecting inositol(1)phosphate, a major product of the phosphatidyl inositol cascade.
“For a given receptor, the same Tag-lite cell line can be used as a starting basis for these tests, which have all been streamlined and validated with prelabeled frozen cells,” stated Francois Degorce, director of marketing and communication.
“Therein lies the breakthrough of the Tag-lite concept—enabling receptor investigation to address multiple angles while eliminating the need to develop different cells for each.”