ProSwift Monolith Columns
Polymer monoliths, a new generation of separation media, provide a solution for this problem. Dionex’ (www.dionex.com) ProSwift™ monolith columns constitute a new family of high-resolution columns for biomolecule analysis. ProSwift columns comprise rigid, porous polymer-based monolithic columns in various formats, from nano to analytical, with diverse chemistries including reversed and ion-exchange phases. These columns feature unique monolith morphology, engineered to contain an uninterrupted, interconnected network of channels of specific, controlled size range. These large flow-through channels allow fast mass transfer even for large biomolecules.
The structure of monoliths favors high-speed separations while maintaining low back pressure (Figure 1). The capacity of monolith ion-exchange media is comparable to that of porous resins, while the resolution is similar to that of nonporous resins. The combination of those two factors gives monolithic columns better overall performance. In addition, ProSwift media exhibit stability over a wide pH range. ProSwift columns can separate and purify proteins, peptides, oligonucleotides, and other biomolecules at high-throughput for characterization studies or for LC/MS analysis.
Previously introduced monolith columns include reversed-phase columns and an anion-exchange column for separation of proteins, peptides, oligonucleotides, and other biomolecules. This tutorial demonstrates applications of two new ion-exchange monolith columns for characterization of proteins.