Low Volume Measurements for DNA, RNA, and Protein
When measuring low volume samples of two microliters or less, variables such as path length (b), concentration (c), and purity become factors that can be easily accounted for using fast, full-spectrum analysis.
As Figure 2 shows, measurements at 230 nm (phenol), 260 nm (DNA/RNA), 280 nm (protein), 340 nm (background), 600 nm (bacteria), and 970 nm (water) can all be taken with one reading. As the inset illustrates, blank correcting for the buffer at 340 nm decreases the standard deviation. This is one example of how this technology allows for lower limits of detection, greater sensitivity, and more reliability in each DNA measurement.