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Mar 1, 2009 (Vol. 29, No. 5)

Measuring Multiplexed Signal Transduction

Amnis’ Platform Was Developed to Assist in Assessment of NF-kappa-B Translocation

  • The common goal of many drug discovery efforts is the modulation of immune responses, for example by the inhibition of tumor necrosis factor-alpha (TNF-a). TNF-a is a potent activator of many cells of the immune system and plays a large role in the pathogenesis of asthma and of autoimmune diseases such as rheumatoid arthritis. TNF-a mediates its pro-inflammatory effect by inducing translocation of NF-kB from the cytoplasm to the nucleus.

    In this article, we present a multiplexed method for the measurement of NF-kB translocation in THP1 cells, a monocytic cell line, in response to TNF-a stimulation at various doses and exposure times. The Amnis ImageStream platform employed in this study images cells directly in flow, making it well-suited to the analysis of both primary and cultured immune cells. Imaging flow cytometry generates over 100,000 cell images per minute to achieve high assay robustness even with rare sub-populations.

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Scientifically Studying Ecstasy

MDMA (commonly known as the empathogen “ecstasy”) is classified as a Schedule 1 drug, which is reserved for compounds with no accepted medical use and a high abuse potential. Two researchers from Stanford, however, call for a rigorous scientific exploration of MDMA's effects to identify precisely how the drug works, the data from which could be used to develop therapeutic compounds.

Do you agree that ecstasy should be studied for its potential therapeutic benefits?

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