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April 01, 2009 (Vol. 29, No. 7)

Measurement of Global DNA Methylation

ELISA-Based Method Simplifies Process and Provides Results in HTS Format

  • Future Work

    While the Imprint Methylation DNA Quantification kit is useful in distinguishing relative levels of global DNA methylation, LC-MS is considered the gold standard for making absolute measurements.  Work is under way to convert this relative, ELISA-based method to an absolute quantitation technique that will be comparable to LC-MS. Samples to be analyzed include SssI methylated DNA, normal human genomic DNA, tumor DNA, and DNA from various cell lines. The data generated from these comparisons is expected to provide a correlation coefficient between LC-MS analysis and our method.

  • Conclusion

    Until now, measurement of global DNA methylation could be cumbersome and often, cost prohibitive. We have demonstrated that our ELISA-based method, the Imprint Methylated DNA Quantification kit, simplifies the process and provides results in about half a day in a high-throughput format. Our method easily distinguishes between methylated and unmethylated DNA. 

    Methylated DNA can be detected in a mixed population of methylated and unmethylated DNA. The signal generated by this method is proportional to the amount of methylated DNA in the sample. DNA from biological systems such as cultured cells, normal tissue, and tumors have also been analyzed. The global methylation levels determined with our method correspond with data generated by other methodologies.

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