Until now, measurement of global DNA methylation could be cumbersome and often, cost prohibitive. We have demonstrated that our ELISA-based method, the Imprint Methylated DNA Quantification kit, simplifies the process and provides results in about half a day in a high-throughput format. Our method easily distinguishes between methylated and unmethylated DNA.
Methylated DNA can be detected in a mixed population of methylated and unmethylated DNA. The signal generated by this method is proportional to the amount of methylated DNA in the sample. DNA from biological systems such as cultured cells, normal tissue, and tumors have also been analyzed. The global methylation levels determined with our method correspond with data generated by other methodologies.