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Aug 1, 2011 (Vol. 31, No. 14)

Mass Spectrometry Systems and Tools Launched at ASMS

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    Beckman Coulter’s CESI 8000 is based on a new front-end separation and ionization technology that fully integrates capillary electrophoresis (CE) with electrospray ionization (ESI) within the same device.

    Beckman Coulter launched the CESI 8000 instrument, which combines capillary electrophoresis and electrospray ionization technology, at ASMS. The system is based on a new frontend separation and ionization technology called CESI, which integrates capillary electrophoresis with ESI in one device. It incorporates OptiMS technology and provides a peak capacity greater than 300. The nanoscale flow leads to reduced ion suppression and higher sensitivity according to the company.

    The Ionics 3Q Molecular Analyzer™ triple quadrupole MS system contains an interchangeable dual probe ESI/APCI source. Ionics collaborated on a poster with Hexagram Analytics that demonstrated efficient single injection analysis of a mixture of chemically diverse pesticides. In the method presented, the flow from the HPLC instrument was split into two flow streams, each providing continuous sample flow to each ion source. In this way users can rapidly switch from one to the other ion source during a single sample injection.

    A poster presentation by Bio-Rad Laboratories compared an in-gel proteolytic digest separation method using the company's soon-to-be launched PROTEAN i12 isoelectric focusing instrument to conventional off-gel peptide fractionation methods.

    With in-gel separation the digest is incorporated into an immobilized pH gradient (IPG) gel by rehydration and is fractionated within the gel matrix. The results demonstrated a 20% increase in identifiable peptide and 14% greater protein identifications, as well as threefold faster separations (excluding elution time), as in-gel IEF can be run at higher voltages than off-gel IEF; however, in-gel methods require additional elution steps. The new instrument provides independent control of individual IEF strips, allowing users to employ more than one pH gradient or voltage setting and run multiple samples in a single experiment.

    The nanoLiter Cool Wave® induction-based dispenser shoots 100% of a nanoliter-scale liquid sample, as a droplet, into a TOF mass spectrometer, according to the company. The device can dispense into an ESI instrument or deposit a sample onto a MALDI source.

    Glygen collaborated on a poster at ASMS that illustrated the use of its Lab-in-a-Tip™ embedded micro-SPE cartridge technology (in the form of its PepTip™ pipette tips) for performing peptide synthesis, with a yield of about 10 nmol of peptide from each tip with up to 98% purity. Glygen also offers is micro-SPE technology in a microspin column format. Integration with an automated liquidhandling system allows for synthesis of 384 solubilized peptides at one time.

    Biotage recently introduced the Pressure+ 96 solid-phase extraction instrument, which processes 96-well plates, and the Pressure+ 48 for processing 48 SPE columns simultaneously. The units employ positive pressure to perform SPE in syringe-based cartridges for sample purification and enrichment upstream of LC-MS.

    Sage-N-Research recently announced an agreement with the U.S. Army Edgewood Chemical Biological Center to commercialize a platform for rapid detection and identification of pathogenic microorganisms in fluids. The platform combines the Sage-N-Research Sorcerer™ computational analysis system and MS-based proteomics methods.

    Covaris is developing sample-preparation kits for proteins designed for use on its S220 system, developed initially for shearing DNA. The isothermal, noncontact sample-prep system utilizes adaptive focused acoustic technology, and all processing takes place in an enclosed vessel.

    Denator featured its new Stabilizor™ Peptide Extraction Kit at ASMS, designed for recovery of water-soluble endogenous peptides from tissue samples. Following heat stabilization to inactivate proteolytic enzymes, homogenization and filtration steps remove molecules greater than 10kDa in preparation for LC/MS analysis.

    The Genedata  Expressionist® 7.0 for mass spectrometry is an enterprise software package for managing, analyzing, and visualizing MS data. Built on an open client-server architecture, the system integrates multiple proteomics and metabolomics workflows. The 7.0 supports MRM for proteomics. Other enhancements include unicode support of special characters and improved handling of multiplex experiments.

    CovalX has expanded its CRO services to offer epitope (linear or conformational) characterization using MALDI-MS for studying protein-protein interactions.

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