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Aug 1, 2011 (Vol. 31, No. 14)

Mass Spectrometry Systems and Tools Launched at ASMS

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    AB Sciex’ SelexION™ technology device is an ion mobility spectrometry tool for improving data quality in the quantitation and characterization of challenging samples.

    Thermo Fisher Scientific premiered three new systems on the Orbitrap™ platform that will reportedly position it to compete in the QTOF market. The Orbitrap Velos Pro™ linear ion trap incorporates a collision cell with axial field gradient, Generation II ion optics, Trap HCD fragmentation, and a high dynamic range of detection for enhanced quantification.

    Thermo optimized the features of the Velos Pro for metabolite identification and peptide sequencing applications. The new Orbitrap Elite Quantum offers resolution greater than 240K FWHM at m/z 400 and four times the scan speed of the Velos model. It can perform parallel MS and MSn analysis and was designed to yield maximal protein identification for omics research.

    The Orbitrap Q Exactive™ is a quadrupole LC/MS/MS system with the quadrupole positioned in front of the Orbitrap. It offers protein identification, quantification, and confirmation at high resolution in a single analytical run, according to the company. The maximum scan speed is 12 Hz, resolving power is up to 140,000 FWHM, and the system features an intra-scan dynamic range greater than 5,000:1.

    Thermo also introduced the EASY-nLC 1000 split-free nano-flow UHPLC system that includes 50 µm UHPLC columns packed with 1.9 µm beads.

    AB Sciex  launched its SelexION™ technology for quantitative and qualitative analysis using differential ion mobility spectrometry (DMS). It can be used for multiple reaction monitoring (MRM), with 25 ms cycle times per MRM. Designed to enhance selectivity to facilitate applications requiring separation of isobaric species, isolation of co-eluting contaminants, and elimination of high background noise, the technology can improve data quality and accelerate sample preparation, according to the company.

    The additional level of selectivity is based on the shape of a molecule. The DMS technology is compatible with AB Sciex' TripleQuad™ 500 System and QTRAP® 5500 System mass spectrometers. The company also introduced SWATH™ Acquisition, a new MS-based technique designed to yield quantitative results for every peptide in a proteomics sample analysis. As part of its MRMAtlas collaboration with ETH Zurich's Ruedi Aebersold, AB Sciex is developing MZS/MSALL functionality to enable SWATH Acquisition on its TripleTOF™ 5600 system.

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    Agilent Technologies’ 6500 iFunnel Q-TOF LC/MS reportedly provides superior sensitivity and data quality for profiling, identifying, characterizing, and quantifying compounds.

    Agilent Technologies introduced the 6550 iFunnel quadrupole time-of-flight (Q-TOF) LC/MS system, which offers low femtogram sensitivity, acquisition rates up to 50 spectra/sec, and up to five orders of intra-scan dynamic range.

    The company's new 6420 triple quadrupole LC/MS replaces the 6410 model and is designed to be readily upgradable to offer the twofold greater sensitivity of the 6430 and the eightfold greater sensitivity of the 6460. Agilent also highlighted its new Mass-Hunter software, which includes a translator system for transferring data into MassHunter.

    Waters introduced the Synapt® G2-S mass spectrometer at the ASMS conference. The G2-S is reportedly 30 times more sensitive than the Synapt G2, providing improved signal intensity and a five times increase in signal-to-noise ratio and tenfold enhanced limit of quantitation. The instrument combines Waters' StepWave™ ion transfer optics and Triwave® ion mobility technologies; ions are separated based on size, shape, and charge before they undergo mass analysis.

    The advantage for drug discovery is that two proteins with an identical mass but different shapes—perhaps because one failed to fold properly—can be distinguished from each other, according to the company. Improper folding may mean the difference between a therapeutic effect and toxicity.

    The G2-S supports Waters' High-Definition MSE acquisition method. Waters' Acquity UPLC I class offers 50% lower dispersion (reducing injection-to-injection carryover), nearly double the peak capacity, higher resolution, and faster run-times than the Acquity H series. Also newly introduced by Waters at ASMS was the Xevo® TQD tandem quadrupole mass detector, which has the same universal ion source architecture as the Synapt G2-S, as well as ZSpray™ and the company's Radar ™ data-acquisition technology.

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    According to Bruker Daltonics, the maXis™ UHR-TOF features mass accuracy and resolution enhancements, and significant sensitivity and speed.

    Bruker Daltonics introduced the benchtop maXis impact™ UHR-TOF MS, with >40,000 FWHM resolution, MS and MS/MS accuracy <1 ppm, ultrahigh pressure LC compatibility, and an acquisition rate of up to 50 full spectra/sec.

    The company's new CaptiveSpray™ LC/MS source combines nanospray sensitivity with the ease of use of electrospray technology and is compatible with 50–5,000 nL/min LC flow rates. The source fits all current Bruker ESI/MS systems. The CaptiveSpray incorporates the company's Etch Taper™ capillary spray tip, which creates a vortex around the spray that concentrates and targets the ions to the MS inlet.

    Shimadzu presented new applications for its ultrafast MS (UFMS) technology, made possible by coupling the company's LC/MS-8030 triple quadrupole MS system to the Nexera UHPLC instrument at the front-end. The system offers sample injection as fast as seven seconds and repeatability of 0.5% in 1 µL injections. Shimadzu also introduced the SIL-30ACMP multiplate autosampler, designed for use with the Nexera UHPLC instrument.

    PerkinElmer launched the AxION™ mass spectrometry platform, which includes software for method selection and set-up, data management, and data analysis. The system contains the AxION 2 Time-of-Flight (TOF)-MS instrument with an Ultraspray™ 2 ESI or Field-Free Atmospheric Pressure Chemical Ionization source. Each ion source comes with interchangeable snap-in probes for easy switching from user to user. The system has a mass range of 18–12,000 m/z, mass accuracy less than 2 ppm at 1,000 m/z, resolution of greater than 12,000 FWHM at 922 m/z, and a linear dynamic range of 105.

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