Thermo Fisher Scientific premiered three new systems on the Orbitrap™ platform that will reportedly position it to compete in the QTOF market. The Orbitrap Velos Pro™ linear ion trap incorporates a collision cell with axial field gradient, Generation II ion optics, Trap HCD fragmentation, and a high dynamic range of detection for enhanced quantification.
Thermo optimized the features of the Velos Pro for metabolite identification and peptide sequencing applications. The new Orbitrap Elite Quantum offers resolution greater than 240K FWHM at m/z 400 and four times the scan speed of the Velos model. It can perform parallel MS and MSn analysis and was designed to yield maximal protein identification for omics research.
The Orbitrap Q Exactive™ is a quadrupole LC/MS/MS system with the quadrupole positioned in front of the Orbitrap. It offers protein identification, quantification, and confirmation at high resolution in a single analytical run, according to the company. The maximum scan speed is 12 Hz, resolving power is up to 140,000 FWHM, and the system features an intra-scan dynamic range greater than 5,000:1.
Thermo also introduced the EASY-nLC 1000 split-free nano-flow UHPLC system that includes 50 µm UHPLC columns packed with 1.9 µm beads.
AB Sciex launched its SelexION™ technology for quantitative and qualitative analysis using differential ion mobility spectrometry (DMS). It can be used for multiple reaction monitoring (MRM), with 25 ms cycle times per MRM. Designed to enhance selectivity to facilitate applications requiring separation of isobaric species, isolation of co-eluting contaminants, and elimination of high background noise, the technology can improve data quality and accelerate sample preparation, according to the company.
The additional level of selectivity is based on the shape of a molecule. The DMS technology is compatible with AB Sciex' TripleQuad™ 500 System and QTRAP® 5500 System mass spectrometers. The company also introduced SWATH™ Acquisition, a new MS-based technique designed to yield quantitative results for every peptide in a proteomics sample analysis. As part of its MRMAtlas collaboration with ETH Zurich's Ruedi Aebersold, AB Sciex is developing MZS/MSALL functionality to enable SWATH Acquisition on its TripleTOF™ 5600 system.