Environmentally Friendly Purification Technologies
There is an increasing demand for cleaner' purification approaches, which minimize the use of materials that affect the performance of the finished product and are a source of harmful contaminants. This not only ensures that the product is safe but also reduces the costs associated with downstream processing.
Researchers working at Invitrogen in Scotland developed a technology that purifies nucleic acids without the use of centrifuge, vacuum, chaotropic salts, solvents, or ethanol that often inhibit downstream enzymatic reactions.
The technology uses a simple linkage that enables the binding and release of nucleic acids, which is mediated by changing the pH of the solution. This method promises to provide performance and process advantages over current purification technologies that involve the use of chemicals. A favorable cost-benefit ratio is expected to push this technology into a variety of applications such as genotyping, expression profiling, cloning, sequencing, short tandem repeat (STR) analysis, and PCR, among others.
Researchers from Boehringer Ingelheim (Ingelheim, Germany) and BIA Separations (Ljubljana, Slovenia) are using an environment-friendly chromatographic technology for the purification of pDNA that allows for the fast processing of large volumes while improving product quality.
Since this method does not require the use of enzymes and organic solvents that are necessary in the conventional alkaline cell lysis method, it avoids quality, patient safety, and environmental-related issues. Apart from purifying pDNA, this technology can also be applied in separation and purification of peptides, proteins, oligonucleotides, and polynucleotides.