At the conference, Amy L. Altman, Ph.D., director of extramural research office for Luminex, discussed the development of xMAP® technology. This technology, she explained, “enables laboratories to multiplex bioassays, reducing time, labor, and costs over traditional methods.” Thanks to its open-architecture platform, xMAP technology can be applied across a wide range of markets.
“Our xMAP technology is the underlying foundation for all our systems,” Dr. Altman said, including the recently released Flexmap 3D system, with 500-plex capabilities for high-volume academic and pharmaceutical research applications.
The smaller, deployable MagPix biological analyzer is for academic research labs and decentralized diagnostic laboratories. The MagPix is a low-cost, rugged, compact, biological testing device designed as a deployable biological analyzer. The MagPix instrument simultaneously detects up to 50 analytes in a single reaction. Because it can include multiple signatures per target agent, false positives are greatly reduced, Dr. Altman pointed out.
“In addition, each microsphere essentially represents a single reaction. Reading up to 100 microspheres per reaction further validates the result by allowing for statistical analysis of each sample.”
A 96-well plate can be analyzed within one hour, Dr. Altman said, and can take advantage of an extensive menu of assay panels built on Luminex xMAP beads. “This bead-based format allows for improved kinetics compared to planar arrays.” Furthermore, “the ability to multiplex both proteins and nucleic acids saves the end-user time, labor, and sample, and is a flexible and scalable solution.”
Rather than interrogating analytes via a flow cell illuminated with focused laser excitation light, “the MagPix employs light-emitting diodes and a charged coupled device imager, coupled with an enhanced magnetic microsphere-based array,” she explained. “The design is rugged with no need for complex laser alignment or hydrodynamic focusing.”