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February 15, 2014 (Vol. 34, No. 4)

Low Endotoxin Plasmid Production from E. coli

Transfecting Mammalian Cells without Endotoxin Cleanup Steps

  • In drug discovery research, E. coli is commonly used for plasmid production for downstream mammalian transfection and protein expression experiments. However, this method usually requires endotoxin cleanup steps prior to mammalian cell experiments to avoid cell toxicity and poor transfection efficiencies. Lipopolysaccharide (LPS) from E. coli , also ...

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