GEN Exclusives

More »

Assay Tutorials

More »
Sep 15, 2009 (Vol. 29, No. 16)

Live-Cell Luminescent Assays for GPCR Studies

Combination of Sensitive Detection and Real-Time Analysis Expands Applications

  • Advantages of the GloSensor cAMP Assay for High-Throughput Screening and Compound Profiling

    • Extremely sensitive—Interrogation of endogenous Gs- and Gi-coupled receptors, direct detection of inverse agonists
    • Huge signal-to-background ratio, up to 500-fold increases in light output
    • Easily miniaturized assay format, including uHTS
    • Low cost in a variety of formats (1-100 µL final assay volume)
    • Glow-type bioluminescent readout; no fluorescence-based interference issues
    • Enables combination screens for agonists, allosteric modulators and/or antagonists
    • Frozen-cell compatible
    • Single-cell imaging using luminescence microscopy; whole-animal imaging studies in progress


Related content


GEN Jobs powered by connects you directly to employers in pharma, biotech, and the life sciences. View 40 to 50 fresh job postings daily or search for employment opportunities including those in R&D, clinical research, QA/QC, biomanufacturing, and regulatory affairs.
More »

Be sure to take the GEN Poll

The Triple Package and Success

One theory for explaining “success," put forward by Amy Chua Jed Rubenfeld, posits cultural traits such as a superiority complex, personal insecurity and impulse control. Union College professors Joshua Hart and Christopher Chabris counter that intelligence, conscientiousness, and economic advantage are the most likely elements of success, regardless of ethnicity. Do you think that Hart-Chabris make a better argument for achieving success than the Chua-Rubenfeld theory?

More »