Advantages of the GloSensor cAMP Assay for High-Throughput Screening and Compound Profiling
• Extremely sensitive—Interrogation of endogenous Gs- and Gi-coupled receptors, direct detection of inverse agonists
• Huge signal-to-background ratio, up to 500-fold increases in light output
• Easily miniaturized assay format, including uHTS
• Low cost in a variety of formats (1-100 µL final assay volume)
• Glow-type bioluminescent readout; no fluorescence-based interference issues
• Enables combination screens for agonists, allosteric modulators and/or antagonists
• Frozen-cell compatible
• Single-cell imaging using luminescence microscopy; whole-animal imaging studies in progress