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Sep 15, 2009 (Vol. 29, No. 16)

Live-Cell Luminescent Assays for GPCR Studies

Combination of Sensitive Detection and Real-Time Analysis Expands Applications

  • Advantages of the GloSensor cAMP Assay for High-Throughput Screening and Compound Profiling

    • Extremely sensitive—Interrogation of endogenous Gs- and Gi-coupled receptors, direct detection of inverse agonists
    • Huge signal-to-background ratio, up to 500-fold increases in light output
    • Easily miniaturized assay format, including uHTS
    • Low cost in a variety of formats (1-100 µL final assay volume)
    • Glow-type bioluminescent readout; no fluorescence-based interference issues
    • Enables combination screens for agonists, allosteric modulators and/or antagonists
    • Frozen-cell compatible
    • Single-cell imaging using luminescence microscopy; whole-animal imaging studies in progress


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Scientifically Studying Ecstasy

MDMA (commonly known as the empathogen “ecstasy”) is classified as a Schedule 1 drug, which is reserved for compounds with no accepted medical use and a high abuse potential. Two researchers from Stanford, however, call for a rigorous scientific exploration of MDMA's effects to identify precisely how the drug works, the data from which could be used to develop therapeutic compounds.

Do you agree that ecstasy should be studied for its potential therapeutic benefits?

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