Label-Free Protein Assays for Process Development, Kinetic Characterization, Protein Folding, and Crystallization Screens

• Broadcast Date: Wednesday, March 28, 2012
• Time: 11:30 AM ET, 8:30 AM PT

REGISTRATION IS FREE

AVAILABLE ON DEMAND

Label-free techniques for detecting and quantitating biomolecular interactions greatly facilitate such analyses by eliminating the need for labeling reagents and providing significant time savings and flexibility in today’s laboratory. Bio Layer Interferometry (BLI) as embodied in ForteBio’s Octet and BLItz platforms extends the capabilities of label-free analysis further, allowing crude samples, sample volumes as low as 4 uL, and high-throughput analysis in applications including protein-protein binding, quantitation, affinity, and kinetics.

Participants in this webinar will discuss multiple applications based on a standard microplate Dip and Read™ label-free format that is widely used in industry and academia today. These applications include improved selection of therapeutic mAbs against viral targets based on kinetic characterization, development of an assay --the Binding Rate Screen-- for screening crude lysates for appropriate recombinant protein constructs, and monitoring protein folding and misfolding.

In this webinar you will learn:

• How label-free Dip and Read assays are used in high-throughput to detect protein folding and unfolding events.
• How label-free assays can rapidly identify recombinant protein constructs expressing variations of the target protein, and prioritize them on a time scale of minutes.
• How BLI technology has been used for protein A quantitation, binding characteristics, and mechanism of action experiments in the selection of therapeutic antibodies.
• How BLI technology can be used to monitor protein-folding events in a variety of protein-based experiments, including detection of small molecules that correct folding for a cystic fibrosis protein and detection of a conformational change of a component of anthrax toxin.

Who Should Attend:

• Molecular biologists
• Process development scientists
• Protein engineering and structural biology scientists
• Protein biochemists working in protein and antibody therapeutics
• Recombinant protein production scientists and process development scientists
• Therapeutic antibody development scientists
• Small molecule drug development scientists
• Scientists in academia working on protein-protein and protein-small molecule interactions

Panelists Include

• Marijn van der Neut Kolfschoten, Ph.D., Senior Scientist, Crucell, The Netherlands
• Mark T. Fisher, Ph.D., Professor, Department of Biochemistry and Molecular Biology, University of Kansas Medical Center
• Jiamin Yu, Ph.D., Senior Scientist, diaDexus

Tamlyn Oliver, Managing Editor, Genetic Engineering & Biotechnology News, will be the host of this webinar. A live Q&A session will follow the presentations, offering you a chance to pose questions to our expert panelists.

AVAILABLE ON DEMAND