Academic and industrial laboratories are under pressure to be more productive with limited resources. Laboratory automation is a critical and effective solution to the needs of modern science.
It is difficult to imagine a modern laboratory without at least one piece of automated equipment. Increased throughput and decreased volume dictate the need for efficient robotics and precise connectivity between analysis steps. The former “Lab Automation” conference, now known as SLAS, takes place in San Diego next month and will spotlight the breakthroughs that push the boundaries of laboratory development processes.
Last year, the Association for Laboratory Automation merged with the Society for Biomolecular Screening (SBS), launching the Society for Laboratory Automation and Screening (SLAS). SLAS is dedicated to advancing scientific research and discovery through laboratory automation and screening technology. Technologies profiled in this article represent just a few highlights from this exciting field.
“Most early microfluidic applications focused on separation and analysis of biochemical molecules,” comments Cristian Ionescu-Zanetti, chief technology officer at Fluxion Biosciences. “However, many cell-based assays also benefit from the advantages of the microfluidic approach, for example applying the shear flow to maintain physiologically relevant conditions or addressing single cells.
“Our development focused on cell-based assays to achieve the throughput and cost of biochemical assays with optimal biological relevance.” Fluxion Biosciences designed several proprietary microplates to enable cell-based assays in the microfluidic format.
“Biomolecular microfluidics trends toward lab-on-the-chip format, where all the valves and processors are integrated on the disposable chip. While this approach provides maximum automation, it also leads to high costs of disposables,” continues Dr. Ionescu-Zanetti.
“We pursued a radically different philosophy of product development: lab-off-the-chip.” Fluxion plates contain a network of passive microfluidic channels, while the controls reside within the external hardware station.
The movement of fluids is driven by air pressure applied to the sample loading chambers. The pressure pushes the sample out of the chamber and through the microchannel. If the cells are bound to the magnetic beads, magnetic capture occurs within the microchannel. The same microchannel may be connected to various other chambers, pneumatically actuated as needed for a particular application.
In addition to cell separation, Fluxion’s applications include cell imaging, automated patch clamping, cell migration assays, drug activity studies, and others.
“Isolation and concentration of circulating tumor cells (CTCs) hold tremendous potential in cancer companion diagnostics,” says Dr. Ionescu-Zanetti. “It is also technologically challenging. Microfluidics enables passing a few cells at a time through the separating region while maintaining the individual cell microenvironment.”
The company’s IsoFlux™ Rare Cell Access System captures the labeled cells on a small polymeric disc that forms the roof of the microfluidic separation channel. When the disc is decoupled from the channel, the cells remain in a small droplet ready for downstream assays.
This combination of microfluidic enrichment and delivery in a small volume opens the doors for continuous cancer monitoring via “liquid biopsy”. While frequent tissue biopsies to monitor cancer recurrence are often not practical, identification of tumor cells via simple blood draw may become routine in clinical patient management.