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Investigating Cell Invasion and Migration

Beyond Endpoint Analysis: Real-Time Monitoring Enables Greater Insight into Cellular Behavior

• Christopher Bird
• ,
• Kairat Madin
• ,
• Shelli Kirstein

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Conclusion

Figure 3. Invasion and migration kinetics of HT1080 cells in indicated dilutions of Matrigel using FBS as the chemoattractant. Click Image To Enlarge +

Figure 3. Invasion and migration kinetics of HT1080 cells in indicated dilutions of Matrigel using FBS as the chemoattractant.

The cancerous cells shown have varying migratory and invasive behaviors, specific to chemoattractant stimuli or Matrigel concentration. The new CIM-Plate 16 for the RTCA DP instrument is a versatile device for the analysis of invasion and migration, and is the only platform capable of quantitatively measuring the invasive and migratory behavior of cells in real time without exogenous labels.

This enables the identification of migratory and invasive time points, which will help increase throughput by shortening assay time, minimize the number of endpoint assays, and help determine optimal time points for inhibitor studies. Moreover, analysis using the xCELLigence System reduces hands-on cell manipulation steps, leading to more physiologically relevant results.