Rainer Heller, Ph.D., is a leader in Greiner Bio-One’s high-throughput screening (HTS) group, where he is responsible for launching many new products dealing with HTS.
Greiner Bio-One boasts newly designed microplates made from cycloolefins. Due to their excellent optical, chemical resistance, and physical properties, cycloolefin microplates have become increasingly popular in research and high-throughput applications, Dr. Heller said.
A variety of different cycloolefin microplates are now, or will soon be, available from Greiner Bio-One for different purposes, including cell-based assays, compound storage, liquid handling, and biochemical assays.
The new 1,536-well SCREENSTAR Microplate, for example, is a cycloolefin microplate designed for microscopic applications, high-content screening, and high-throughput screening. The SCREENSTAR Microplate was co-developed by Greiner Bio-One and GNF Systems and features a black pigmented frame with a 190 µm ultra-clear film bottom for ideal compatibility with instrument optics.
Well bottoms display excellent optical properties for the highest optical transparency, with reduced autofluorescence in the lower UV range, low birefringence, and a refractive index of 1.53, the same as glass. Recessed microplate wells enable complete periphery access for high-magnification objectives. Cell culture treatment and sterility assure exceptional performance, he said, for high-content screening, especially with fluorescence microscopy in the lower UV range.
A smooth microplate top, absent of alphanumeric coding, facilitates flush lid mounting for use with ultra-high-throughput screening systems. A similarly constructed 96-well cycloolefin microplate will soon be available from Greiner Bio-One, and the company is currently developing a similar 384-well cycloolefin microplate.
Another application of cycloolefin microplates is for compound storage, as cycloolefins exhibit low water absorption, low impurities, high transparency, and resistance to polar solvents, particularly DMSO, which is commonly used to preserve biological samples.
In order to make the latest technical and design innovations available for HTS, Greiner Bio-One will soon be introducing a new 1,536-well cycloolefin microplate for compound storage, liquid handling (including acoustic systems and pin tools), and transmission measurements in biochemical assays.
This new 1,536-well microplate will follow the most relevant ANSI recommendations and feature a smooth microplate top, also absent of alphanumeric coding to facilitate flush lid mounting for use with the GNF ultra-high-throughput screening system. The wells are more tapered than in classic 1,536-well microplates, reducing the dead volume in different liquid-handling applications.