Evaporation from Plates. The overall plate evaporation from six different plate types is shown in Figure 2. The presence of the evaporation buffer zone in the Nunc Edge Plate dramatically reduces the overall plate evaporation to less than 1% after four days incubation, or 2% after seven days incubation, in comparison to more than 8% for the other commercially available plates.
Effect of Agarose in the Moat. An evaporation buffer zone consisting of 0.5% agarose provides the same low plate evaporation as water, while providing the solidifying effect required to prevent spillages during automation. Both remain below 2% after seven days incubation, in comparison to over 8% observed with a standard 96-well plate (data not shown).