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March 15, 2014 (Vol. 34, No. 6)

Increasing On-Target NGS Reads

Use of Adapter Blocking Oligos for In-Solution Hybridization Improves Target Capture Performance

  • In-solution hybridization (target capture) has been used since the early days of next-generation sequencing (NGS) to enrich for genomic regions of interest. Hybridization protocols have routinely included Cot-1 DNA, which blocks repetitive sequences to prevent nonspecific binding of hybridization probes. However, researchers continued to experience poor signal-to-noise ratios, diminishing ...

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