The Olympus FSX100™ microscope now has an environmental control option to enable time-lapse imaging of live cells, with the addition of the Tokai Hit® compact Stage Top Incubator. The incubator module provides temperature, humidity, and CO2 control and features a clear glass-heated top plate to prevent condensation. Users can select from control units that provide only temperature control, premixed 5% CO2, or integration with a digital gas mixer for 100% CO2 delivery. Olympus also featured its self-contained FV10i confocal-scanning microscope with darkroom capabilities.
Tokai Hit’s INU series of compact environmental chambers offer a range of adaptors to accommodate a 35 mm culture dish, a 50/60 mm dish, chamber slide, chambered cover glass, or slide glass. The company designed the Stage Heater to minimize anti-Z-axis drift and maintain optimal focus for confocal applications as well as time-lapse and long-term imaging experiments.
UVP introduced the new iBox Explorer fluorescence imaging microscope designed for preclinical in vivo imaging studies in small animals. Cancer-related applications include the study of tumor shedding and metastasis, primary tumor growth, angiogenesis, hematogenous trafficking, and interactions at tumor margins. The iBox Explorer detects fluorescent markers in the visible to near-infrared range. It includes configurable filters and generates a continuous excitation spectrum. The motorized stage repositions the animal across the X,Y, and Z axes. Motorized optics adjust the field of view to allow for imaging of whole organs down to single cells.
Thorlabs previewed a prototype of the MZS350 piezo-driven Z-axis scanning stage that will be available in spring 2011. It is capable of 350 µm of travel in the Z direction and allows for repeatable positioning for rescanning of a sample with a high degree of accuracy. Applicable for confocal microscopy and 3-D imaging, the MZS350 is compatible with the company’s MLS203 Series of XY stage systems.
Cellasic featured its Microfluidic 3D Culture Array (MiCA) technology, which allows for 3-D perfusion culture in a gel matrix in a 96-well format. Thirty-two independent flow units each contain a linked flow inlet well, a cell chamber, and an outlet well. The company designed the cell chamber to model the interstitial tissue environment. Cellasic’s Onix™ microfluidic plates and control system allow for long-term live-cell imaging by utilizing microfluidic technology to maintain favorable environmental conditions to support live-cell perfusion within each chamber.