Non-UF LucraTone Soy P promotes cell growth and protein production of CHO cells: CHO cells were grown in the presence or absence of 1 g/L LucraTone Soy P hydrolysate over a ten-day time course. The presence of Soy P improved both cell growth (Figure 1) and protein production (Figure 2) compared to a media-only control. Similar results were observed with LucraTone Soy F, another non-UF hydrolysate (Figures 1 and 2).
Ultrafiltration further reduces endotoxin levels in soy hydrolysate: With all five soy P samples used in this study, both normal flow and tangential flow ultrafiltration reduced endotoxin to levels that were undetectable by LAL assay. Note that unfiltered LucraTone Soy P hydrolysates yield consistently low endotoxin levels even prior to ultrafiltration.
Ultrafiltered and nonultrafiltered LucraTone Soy P promote similar increases in CHO cell growth and protein production: CHO cells were grown in the presence or absence of 1 g/L LucraTone Soy P hydrolysate over ten days. The LucraTone Soy P was ultrafiltered by normal flow (NF) or tangential flow filtration (TFF) methods, and cell growth in the presence of these filtered hydrolysates were compared to a nonfiltered control. Neither NF nor TFF altered the improved cell growth (Figure 3) and protein production (data not shown) in the presence of LucraTone Soy P.