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Jan 1, 2009 (Vol. 29, No. 1)

Impact of Ultrafiltration of Hydrolysates

Study Shows Process Does Not Improve Cell Culture Performance

  • Results

    Click Image To Enlarge +
    Figure 1. Effect of soy hydrolysates on CHO cell growth

    Non-UF LucraTone Soy P promotes cell growth and protein production of CHO cells: CHO cells were grown in the presence or absence of 1 g/L LucraTone Soy P hydrolysate over a ten-day time course. The presence of Soy P improved both cell growth (Figure 1) and protein production (Figure 2) compared to a media-only control. Similar results were observed with LucraTone Soy F, another non-UF hydrolysate (Figures 1 and 2).

    Ultrafiltration further reduces endotoxin levels in soy hydrolysate: With all five soy P samples used in this study, both normal flow and tangential flow ultrafiltration reduced endotoxin to levels that were undetectable by LAL assay. Note that unfiltered LucraTone Soy P hydrolysates yield consistently low endotoxin levels even prior to ultrafiltration.

    Ultrafiltered and nonultrafiltered LucraTone Soy P promote similar increases in CHO cell growth and protein production: CHO cells were grown in the presence or absence of 1 g/L LucraTone Soy P hydrolysate over ten days. The LucraTone Soy P was ultrafiltered by normal flow (NF) or tangential flow filtration (TFF) methods, and cell growth in the presence of these filtered hydrolysates were compared to a nonfiltered control. Neither NF nor TFF altered the improved cell growth (Figure 3) and protein production (data not shown) in the presence of LucraTone Soy P.

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    Figure 2. Effect of soy hydrolysates on CHO cell IgG production
  • Click Image To Enlarge +
    Figure 3. Effect and variability of five soy hydrolysate lots on CHO cell growth

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