Single-use cell culture systems are the hot topic of the moment, and with good reason. Disposable systems, such as T-flasks, roller bottles, and bags, can offer a variety of advantages over fully instrumented bioreactors. These benefits include elimination of:
Cleaning, as they come presterilized, saving work in the lab, as well as eliminating the need to validate the cleaning process;
Autoclaving, as they are presterilized and ready to go;
Lengthy learning curves, as these systems tend to be plug-and-play simple;
Costly start-up, as there is no need for expensive designs, piped-in steam, water lines, or other utilities;
Cross-contamination issues between batches, as the system is disposed of when done.
Until now, disposables have had many disadvantages. They:
Produce low cell densities and product yields, especially when compared to stirred tank bioreactors;
Occupy a lot of lab space to produce equivalent yields to those of more capable systems;
Are labor-intensiveespecially when you are caring for dozens or hundreds of cultures.
New Brunswick Scientific (NBS; Edison, NJ) has introduced FibraStage, a new, high-yield, disposable cell culture system. The labor-saving system produces high yields of protein, virus, or cell mass from anchorage-dependent or suspension cultures, while offering all the benefits of disposable technology.
The complete system is comprised of up to four disposable FibraStage 500 bottles, a FibraStage console that controls bottle movement, and an NBS or user-supplied CO2 incubator to control temperature and gas exchange.