Economics and Ease of Operation
Modified silica-based protein A media is incompressible, meaning that a 1 L column is packed with 1 L of media. This feature is an improvement over soft gels such as agarose-based media that have shrink factors of 0.75 and require a higher quantity of media to pack a 1 L column.
Moreover, silica-based media is easy to pack and can be used with most chromatography equipment (low, medium, and high pressure) from lab to production scale. For example, the packing of a 20 cm bed length in a 110 mm I.D. low pressure Prochrom® Bio column takes two minutes to obtain 5,900 plates at 160 cm/h.
This comparative study illustrates the benefits of AbSolute modified silica-based protein A media in large-scale mAb production. Its use allows biomanufacturers to speed up their antibody-capture processes, making them more cost-effective.