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March 01, 2011 (Vol. 31, No. 5)

High-Throughput Hybridoma Screening

EMBL Researchers Find that the Arrayjet Super-Marathon Inkjet Microarrayer Can Improve Process

  • Results

    Click Image To Enlarge +
    Figure 1. A comparison of a chip coated with a peptide containing a phosphor-serine (left), with a chip coated with an unphosphorylated version of the same peptide. Spots labeled A are specific to the phosphorylated form and are therefore not present at point B.

    A comparison of images of the three slides in the phospho-specificity test fusion enabled isolation of hybridomas that produced antibodies specific for the phospho-peptide (Figure 1). In addition, a comparison of the microarray images produced by the contact microarrayer with those produced on the Super-Marathon demonstrated the superior spot morphology of the microarrays produced by the Super-Marathon.

  • Click Image To Enlarge +
    Figure 2. A comparison of a typical protein chip spotted with 32 fixed pins (left) with the typical spotting pattern of the Arrayjet Super-Marathon

    The improved microarray quality simplified and accelerated the analysis (Figure 2), while enabling rapid and simultaneous screening of the supernatants from the seven other fusions. Clone selection and isolation were completed within 36 hours of commencing the microarray assay (data not shown).

  • Conclusion

    Robust systems for high-throughput hybridoma production and screening are absolutely necessary in order to produce high-quality antibodies in the most efficient manner possible. The Super-Marathon Inkjet Microarrayer has been shown to improve the speed and robustness of manufacture of the screening assay due to its flexibility, reliability, and the quality of the microarrays it produces.

    In addition, the quality and accessibility of the microarrays produced on the Super-Marathon enabled more rapid data analysis and interpretation. The capacity and flexibility of the Super-Marathon could enable the introduction of new methods, such as epitope-mapping of fusions at the primary screen, particularly useful when attempting to raise matching pairs of monoclonals for sandwich assays such as ELISA, proximity ligation assays, multiplex bead assays, and for protein quantitation.

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