Automated qPCR Setup
The Access workstation easily automates the preparation of qPCR assays in 384- and 1,536-well formats for most detection platforms. Performance of the Access workstation was evaluated for preparation of 1,536-well Roche PCR microplates for qPCR analysis. 100 nanoliters of cDNA along with 500 nanoliters of probes, primers, and master mix was transferred into each well of a microplate following an automated workflow shown in the Table.
After preparation, all plates were cycled using the Roche LightCycler® 1536 System. After analysis, the Access workstation was used to cherry-pick cDNA samples from stock plates, for all reactions with a low copy number, into 1,536-well PCR microplates to create a dilution series for further characterization. Twofold dilutions of higher concentrations of cDNA were used to identify discrete changes in mRNA expression when cycling on the LightCycler.