Quantitative PCR (qPCR) is a powerful tool used for genotyping, SNP analysis, and gene-expression studies. Quantitative detection of small mRNA expression changes facilitates the characterization of early-stage cancer and infectious disease. Advances in qPCR technology to enable the use of high density 384- and 1,536-well microplates have led to a demand for automation and miniaturization of qPCR preparation.
With automation, researchers can generate larger sets of data quickly, while minimizing resource burden and improving reproducibility across assays. As researchers push further toward miniaturization, potential cost savings are met with a high risk for cross contamination, lower precision, and poor accuracy.
Labcyte’s Access™ workstation relies on the company’s Echo® liquid handler and its tipless, touchless transfer of samples and reagents to eliminate such risks. With the use of acoustic energy, the Echo liquid handler enables cherry-picking and reformatting of samples from any well of a source plate to any well of an assay plate with high precision, accuracy, and no risk for cross contamination.
The Access workstation seamlessly adds automated sealing, centrifugation, and bulk reagent dispensing—creating a simple walk-up and walk-away solution to overcome the challenges of miniaturized, high-throughput gene-expression profiling (Figure 1).