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January 01, 2014 (Vol. 34, No. 1)

Genome Editing with CRISPR Systems

Methodological Approach Designed for Ease of Use, Speed, and Low Cost to Clone

  • CRISPR endonucleases have been used in a recent flood of literature due to their elegant and simple mode of RNA-guided gene targeting and ability to operate via protocols developed originally for ZFN-based genome editing in human cells, rats, flies, worms, and plants among other organisms. 1-4 The literature ...

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