Reproducible Controlled-Rate Freezing
CoolCell alcohol-free, controlled-rate cell-freezing containers provide a consistent and reproducible –1°C/minute freezing rate and deliver results comparable to a programmable freezer.
CoolCell containers are made from a closed-cell polyethylene foam that is highly insulative and has superior material properties at cryogenic temperatures. The radially symmetric vial distribution ensures identical heat-removal profiles for each vial while a solid alloy thermal core balances and fine-tunes the freezing profiles, ensuring reproducibility (Figure 2). The single-block base construction is durable and provides nearly indefinite product life-cycle without changes in performance, resulting in a consistent, reproducible, and standardized alternative to programmable freezers for cell cryopreservation.
Recently published papers in Nature Protocols advocate the use of BioCision’s CoolCell alcohol-free controlled-rate freezing container in the preservation, storage, and recovery of sensitive stem cells. According to John Gardner, senior project leader at Roslin Cellab, “controlled freezing with the CoolCell container greatly increased the reproducibility of the freeze process, with increased cell viability and cell growth post thaw.”
In addition, ATCC also now recommends the incorporation of a CoolCell container into their iPSC stem cell handling guide. Kevin Grady, product line business manager at ATCC, says “the device is elegant in its simplicity and ease of use and offers researchers a method to cryopreserve cells in a standardized fashion with great reproducibility and little variability in performance.”
Standardized cryopreservation techniques are also an important aspect of developing cell therapies. Following the expansion and/or manipulation of cells in culture, some cell therapy products are stored in liquid nitrogen until immediately prior to patient administration. A recent independent example of product utilization in the cell therapy space was published in the Journal of Autoimmunity. Researchers cited the use of a CoolCell freezing container for studying the role of T cells in myasthenia gravis. The CoolCell freezing container was used to gradually freeze cells isolated from the blood of both patients and healthy donors for long-term storage while sample collection was underway.