Biotechnology from bench to business

• Follow GEN on:
• Login | Register | Subscribe

Expanding Disposable Depth-Filter Applications

Pall Focuses on the Improvement of Process Clarification Operations

• Scott Anderson
• ,
• Mike Collins
• ,
• Manfred Muehl

(Page 2 of 4)

Test Overview

Figure 2. Comparative filtration capacities for Supracap 100 capsules, Supradisc modules, and Stax capsules (at 1 bart dP) Click Image To Enlarge +

Figure 2. Comparative filtration capacities for Supracap 100 capsules, Supradisc modules, and Stax capsules (at 1 bart dP)

Test Fluid: Homogenized Yeast Test Solution, developed to be representative of a postcentrifuge mammalian cell type process solution.

Depth Filter Medium: Seitz grade EKSP

Filter Formats: Supracap™ 100 capsules, Supradisc™ II 16-inch modules, and Stax capsules (Table, Figure 2)

Sheet Format: Single Layer

Stax Port Configuration: Inlet, bottom of stack; Outlet, top of stack

Feed Flow Rate: Constant at 300  L/m²/hr (typically the maximum flow rate used for biotech applications)

Volumetric Throughput Target: 300 L/m² (typical volumetric throughput value for biotech applications)

Trial Procedure

Filter details Click Image To Enlarge +

Filter details

• Preflush all filters with filtered process water, 50 L/m² at a minimum flow rate of 300 lmh.
• Measure the pressure drop characteristics through all assemblies at flow rates of 100, 200, and 300 lmh.
• Perform filterability trials at a constant feed flow rate of 300 lmh.
• Record time, feed pressure, filtrate pressure, filtrate volume, and turbidity throughout the trial.
• Continue trial until the pressure drop across the filter is a minimum of 1.0 bar (14.5 psi).
• All instrumentation must be calibrated prior to test program.
• Analyze samples at regular intervals for instantaneous turbidity measurement.