Evolutionary vs. Revolutionary Progress
The “classical” view of purification bottlenecks caused by upstream-downstream mismatches retains validity in many cases, at least operationally.
Most bottlenecks occur as a result of equipment or facility limitations, says Sa V. Ho, Ph.D., senior research fellow at Pfizer Biotherapeutic Pharmaceutical Sciences. For example, “if, over time, through optimization of cells and upstream processes, the titer rises from one or two grams per liter to four or five grams, you will find yourself with two or three times as much product to process from the same volume of broth.”
Downstream operations that are limited in terms of capacity or physical layout, or designed specifically for low-titer cell cultures, are the hardest hit.
Within the downstream process itself, specific unit operations tend to be limiting, particularly those already operating at full capacity. Chromatography columns, for example, typically have a limited binding capacity range within which they are optimized for product purity and yield. In cell culture processing, protein A capture resins for monoclonal antibodies have improved in terms of binding capacity, but their expense complicates their efficient utilization.
“You would think that debottlenecking a capture chromatography step would be a simple matter of buying more resin and filling a larger column. But many facilities cannot accommodate much larger columns or the required buffer preparation and storage. Some facilities simply cannot withstand the total weight of a two-meter column, for example, even if resin cost were not a factor.” Dr. Ho explains.
Similarly, managing high downstream expectations in light of upstream productivity is “the current issue” for Thierry Ziegler, Ph.D., who heads bioprocessing and core platform efforts at the Sanofi Vitry, France facility.
Dr. Ziegler holds that “evolutionary” downstream improvements have not kept up with protein titers from cell cultures. “If you look back ten years, tools for mAb purification consisted mainly of protein A, ion exchange, and hydrophobic interaction chromatography. Today the new resins have improved capacity and functionality, but there was no quantum leap in how much protein that could handle. Downstream processing simply has not progressed to the same degree as upstream.”
Resin vendors argue, with justification, that performance improvements have been substantial. Protein A resins bind 50–100% more protein per volume of resin than a decade ago, and improved resistance to cleaning/sanitizing agents allows running more cycles than ever. On the ion-exchange front, new resins, ligands, and matrices have improved capacity, flow rates, and selectivity.
Mixed-mode ligands have been around for many years (the original mixed-mode medium, hydroxyapatite, was discovered in the early 1900s) but have only recently been recognized for their exceptional selectivity and binding capacity, and their potential for reducing process steps.
Mixed-mode columns have enabled Sanofi to compress mAb purification from the traditional three-column process to a two-column scheme consisting of mixed-mode and ion-exchange columns while providing equivalent yield and product purity.
Ion exchange works well as capture and intermediate chromatography for many recombinant, nonantibody proteins because of the wide variation in the proteins’ isoelectric points (pIs). Ion exchange is less effective for monoclonals, but mixed-mode columns are increasingly viewed as alternatives for both capture and impurity removal.
No one is intimating that protein A is on its way out. “Protein A cost is counterbalanced by the very high purity of mAbs they help generate,” says Dr. Ziegler. “What some critics fail to realize is that highly efficient and robust capture means producers need to invest less in operations succeeding protein A capture. If they began with cation exchange, they’d need to invest more in steps downstream of capture.”
Upstream productivity has had far less impact on removing residual impurities than on capture and intermediate chromatography. The throughput of “polishing” columns, which is run in flow-through mode, depends more on impurity levels—which remain relatively low even in high-titer processes—than protein concentrations.
Many companies, Sanofi included, have replaced anion-exchange polishing with membrane chromatography to reduce processing time and lower costs related to buffers and resins. Not every purification is achievable in flow-through mode, and membrane adsorbers are not competitive when used in bind/elute mode.