When it comes to isolating and purifying DNA, RNA, and protein analytes, there are considerable benefits to be gained in getting all three from a single, undivided sample. These benefits include data consistency, sample preservation, and time savings. The ability to build an accurate picture of the relative levels of nucleic acids and proteins in individual tissue and cell samples brings an extra dimension to quantifying expression levels.
GE Healthcare Life Sciences’ illustra™ triplePrep kit can perform all three purifications from a single starting sample, as part of the same protocol, which saves a significant amount of time. In addition, there are potential benefits in how much information you can get from your sample and how best to utilize the available sample material.
The triplePrep kit uses no more sample than would be required to purify a single analyte by conventional methods, so the immediate benefit in reducing the amount of sample required is clear. If samples cannot be replaced easily or are in short supply, then isolating all three analytes in one process is a valuable alternative. Biobanks and sample libraries can be preserved, and hard-to-replace samples are utilized more effectively.
Few samples can be said to be truly homogeneous, even two separate cell culture plates could have different expression profiles. When samples are more complex, or where a more precise understanding of expression profiles is required, the ability to isolate and purify DNA, RNA, and protein from exactly the same starting material is particularly valuable.
Tissue samples, in particular, may contain multiple cell types, and the proportions of each cell type can vary significantly across the sample, making a reliable comparison difficult. One starting sample for all three analytes enables consistent correlation of purified DNA, RNA, and protein.
In this article, triplePrep is compared with traditional, single-analyte, purification kits. Under these experimental conditions, the results indicate no adverse effect on either yield or purity, and improved yields in some instances.