The use of porous or nonporous suspended microcarriers in traditional stirred-tank or airlift bioreactors makes it possible to increase the available growth area for anchorage-dependent cells. Recently, ATMI LifeSciences (www.atmi-lifesciences.com), Artelis (www.artelis.be), and Pierre-Guerin Biolafitte (www.biolafitte.com) teamed up to develop scalable, disposable bioreactors to meet this need.
This new scalable bioreactor features noninvasive paddle-mixing technology developed by ATMI, standard DO, pH, and temperature probes with a Tryton® monitoring device from Pierre Guerin Technologies, and is made out of materials compatible with the growth and viability of animal cells. Preliminary tests, set-up, and cultures were performed by Artelis.
The paddle-mixing system provides gentle agitation to protect animal cells growing on the microcarriers at high cell densities. The bioreactor is able to agitate the cultures without causing bead-to-bead shock that can damage the cells, while a microsparger fixed to the paddle ensures oxygenation (Figures 1A and B).