The Maxwell 16 System is designed to allow multiple reagent chemistries to run on a single instrument. The Maxwell 16 gDNA Isolation Kits have been validated for use with whole blood (400 µL), tissue culture cells (5x106), and a variety of whole organism and tissue types (Figure 2a). The unique design of the Maxwell 16 Plunger allows the direct processing of a variety of solid sample types such as whole Drosophila flies, animal and plant tissues, and rodent tails without preprocessing.
Once samples are placed into prefilled reagent cartridges, the cartridges are loaded into the instrument, and an automated method is selected. Purified gDNA is delivered in a concentrated format ready for downstream analysis without the need for differential lysis, fractionation of white cells, centrifugation, precipitation, or rehydration of gDNA prior to analysis. Yield and performance in downstream applications such as PCR are shown in Figure 2b.
The Maxwell 16 Total RNA Isolation Kit can be used with a variety of sample sources and is effective at isolating RNA from samples containing a wide range of total RNA content. For most tissue types up to 50 mg of sample can be processed. The system uses a proprietary clearing agent to effectively remove contaminating gDNA without using a nuclease, and fewer than 0.1 genomic equivalents are present per 100 ng of isolated RNA as determined by qPCR analysis. Most tissue types can be prepared for purification by disruption in RNA Lysis Buffer using mechanical shearing.
We have also isolated total RNA from stabilized blood, the white blood cell fraction of whole blood, and plant leaf tissue. Yields are excellent with A260/A280 ratios of 2.0 or better. The isolated RNA is of high quality, and performance in downstream applications is excellent (Figure 3).
The Maxwell 16 Polyhistidine Protein Purification kit is designed to isolate recombinant polyhistidine- or HQ-tagged proteins from bacterial cells, mammalian cells, insect cells, or tissue culture medium (Figure 4). Sample or lysate is added directly to the reagent cartridge, and purified proteins are obtained in about 40 minutes of hands-free operation. Once purified, proteins are ready for downstream analysis using a variety of techniques such as polyacrylamide gel analysis, Western blot, MALDI-TOF mass spectrometry, and functional analysis.