Virus-like particles (VLPs) are a relatively new area within vaccine development. They have been designed to overcome the problems associated with certain recombinant protein vaccines that have poor immunogenicity resulting from poor presentation of the viral antigens to the immune system. This can be overcome to a certain extent through the addition of adjuvants, but perhaps another more attractive option is available through the creation of VLPs.
VLPs consist of an assembled structure of viral antigens creating a more authentic structure and conformation of the viral antigen. VLPs have been shown to have significant potential in eliciting a stronger and lengthier immune response than more traditional recombinant protein vaccines.
In terms of characterizing such structures, infectivity assays cannot be used as VLPs, are devoid of the RNA or DNA required for replication, and hence, they are noninfectious. In addition, they cannot be quantified using qPCR again due to the fact that they do not contain DNA or RNA. The NanoSight technique does not require the particle to be infectious nor contain DNA/RNA and hence represents an attractive method of characterizing these structures.
The NanoSight technique can be used as a tool to quantify the total viral content and can be used alongside infectivity assays to calculate the relative concentrations of infectious to noninfectious viruses in a live attenuated vaccine. This method is ideally suited to calculate viral titers in inactivated vaccines where infectivity assays cannot be used to characterize the final product.